Extended Data Fig. 9: Comparison of NTSR1-Gi interaction in lipid bilayer with detergent micelles.
From: Cryo-EM structure of an activated GPCR–G protein complex in lipid nanodiscs
a-c, Superposed structure of C-state NTSR1 (blue) and Gα (gold) in cND, NC-state NTSR1 (orchid) and Gα (dark cyan) in cND, C-state NTSR1 and Gα in micelle (gray, PDB 6OS9), NC-state NTSR1 and Gα in micelle (magenta, 6OSA). The models are superposed on NTSR1. a, extracellular view of NTSR1 and αN-helix; b, side view of NTSR1 ICL3 and α4β6 loop; c, side view of NTSR1 and α5-helix. d, Comparison of the localization of α5-helix relative to GPCR in class A GPCR-Gi complex structures, including the canonical (gold) state and noncanonical (dark cyan) state structure reported in the current study, canonical (gray) and noncanonical (magenta) state of NTSR1-Gi in detergent micelle, µOR-Gi (lime green; PDB 6DDE), A1R-Gi (cyan; PDB 6D9H), CB1-Gi (purple; PDB 6N4B), Rho-Gi (hot pink; PDB 6CMO) and DRD2–Gi (yellow; PDB 6VMS). The structures are superposed on the GPCR. Residue R3.50 is shown as colored spheres in C-state NTSR1 and as partially transparent gray spheres in the other GPCRs.
