Extended Data Fig. 6: RNA processing gene knockouts sensitize cells to LINE-1.

(a) StringDB network plot of the 81 mRNA processing genes identified by this screen. Edges indicate known protein-protein interactions. This network is enriched for spliceosome machinery (green nodes). (b) Screen behavior of significant genes belonging to the spliceosome KEGG GO term. Median sgRNA counts are depicted with 95% Confidence Intervals. (c) Clonogenic assay (12 d) comparing growth of luciferase⁺ and LINE-1⁺ cells (induced with 1 µg ml^–1 doxycycline) treated with 1 nM pladienolide B (PLA-B) or vehicle (DMSO). n = 3 independent experiments. Error bars indicate s.e.m. P value calculated by unpaired one-sided t-test. (d) Behavior of nuclear exosome complex genes in the screen. Median values are depicted with 95% Confidence Intervals. (e) Behavior of RNASEH2 component sgRNAs in the screen. Median values are depicted with 95% Confidence Intervals. (f) Behavior of ADAR1 sgRNAs in the screen. Median values are depicted with 95% Confidence Intervals.