Extended Data Fig. 1: Biochemical characterizations of recombinant mBcs1.
From: Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein
a, Size exclusion chromatographic profile of mBcs1 on Superdex 200. Inset: SDS-PAGE showing the purity of mBcs1 along the purification steps. b, One representative Michaelis Menten plot out of three experiments of mBcs1 ATPase activity to ATP concentration (n=3 technical replicates). c, Nucleotide-dependent conformational change of mBcs1 in the presence of different nucleotides as observed by Blue-Native PAGE.
