Extended Data Fig. 3: Data processing workflow for mBcs1 in ATPγS-bound form. | Nature Structural & Molecular Biology

Extended Data Fig. 3: Data processing workflow for mBcs1 in ATPγS-bound form.

From: Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein

Extended Data Fig. 3

Particles of mBcs1 in the presence of ATPγS appear to form a mix of single and dual heptamer. Initial processing showed the two heptamers in the dual heptamer do not interact uniformly, hence reducing the resolutions. Therefore, only the class that contains single heptamers was selected for subsequent refinement. To improve resolution, the AAA-domain was masked for final refinement.

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