Extended Data Fig. 4: Single-particle cryo-EM analysis of mBcs1 in ATPγS-bound form.
From: Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein
a, Representative motion-corrected micrograph of an ATPγS-bound mBcs1 sample. b, Gold-standard Fourier shell correlation (FSC) curves of the final density map of the ATPγS-bound mBcs1. The reported resolution for this structure was based on the FSC=0.143 criterion. c, Local resolution map of ATPγS-bound mBcs1 structure at 4σ contour level. The color code is given by the bottom color strip. d, Angular distribution of particles used in the final 3D reconstruction of ATPγS-bound mBcs1. The height of the cylinder is proportional to the number of particles for that view. e, Map-to-model FSC.
