Extended Data Fig. 7: The C-terminus of ScMaf1 is not obligatory for Maf1 function.
From: Structural basis for RNA polymerase III transcription repression by Maf1
a, Amino acid sequences of wild-type ScMaf1 and the various ScMaf1 C-terminal mutants are shown from the end of conserved domain C22 through the acidic terminal region (where present). Amino acid sequences from residue 2 through 326 are represented by //. ScMaf1ΔCt terminates at residue 346 while ScMaf1ΔCtSpMAF1 and ScMaf1ΔCtHsMAF1 proteins contain the terminal amino acids from S. pombe (35 residues, colored in blue) and human (45 residues, colored in pink) MAF1 proteins appended to ScMaf1ΔCt. b, The respiratory defect of the maf1Δ::natMX vector only strain, poor growth on glycerol, is rescued by wild-type ScMaf1 and the three ScMaf1 C-terminal variants. Ten-fold serial dilutions of maf1Δ:: natMX strains containing pRS314 vector, pRS314ScMaf1 or pRS314ScMaf1 C-terminal variants grown at 30 and 37 °C on media with glucose (left panels) and glycerol (right panels) as the carbon source. c, Northern analysis of Pol III transcription and repression shows that the C-terminus of ScMaf1 is not required for the Maf1 repression function in yeast. maf1Δ:: natMX strains containing pRS314ScMaf1 or pRS314ScMaf1 C-terminal variants (ScMaf1ΔCt, ScMaf1ΔCtSpMAF1 and ScMaf1ΔCtHsMAF) were treated with rapamycin or drug vehicle for 1 h. The relative level of Pol III transcription is reported by the amount of pre-tRNALeu transcript normalized to U3 snRNA, expressed relative to the untreated wild-type strain and indicated below each lane.
