Extended Data Fig. 2: Cross-seeding αSyn variants using seeds created from WT αSyn.
From: A short motif in the N-terminal region of α-synuclein is critical for both aggregation and function
a, ThT fluorescence assays of αSyn variants (100 μM) WT (black), ΔP1 (red), ΔP2 (green) and ΔΔ (blue) seeded with 10 % (v/v) WT αSyn fibril seeds formed at pH 7.5. Seeding assays were performed at pH 7.5, low salt (20 mM added NaCl), 37 °C, quiescent. b, End point (42 h) TEM images of representative samples of fibrils from the seeding experiments using the same color scheme as in a. Scale bars, 200 nm. Data for graph in a are available as Source data.
