Extended Data Fig. 5: Intramolecular PRE experiment for P1P2-GS αSyn.
From: A short motif in the N-terminal region of α-synuclein is critical for both aggregation and function
a, Intramolecular PRE intensity ratios of amide protons (paramagnetic/diamagnetic) for P1P2-GS αSyn with the MTSL spin label at A90C at low ionic strengths (20 mM NaCl), 15 °C, pH 4.5. Blue, pink and red bars show intensity ratios for residues in the N-terminal, NAC and C-terminal regions, respectively. Dark blue bars highlight residues in the P1 and P2 regions that could be assigned and measured. The gray boxes mark the P1 and P2 regions. Black arrows show only a small PRE effect is observed in the P1-P2 region for P1P2-GS. Due to the repeating glycine and serine residues in the P1-P2 sequence, not all residues could be assigned (Methods). b, Comparison of a rolling window (over five residues for easier comparison) of the PRE effects for WT (blue), ΔΔ (red) and P1P2-GS (orange) αSyn. The black box is zoomed out in c to highlight residues in the P1-P2 region. The data for WT and ΔΔ are shown in Figs. 4d and 5d. Data for graphs in a,b are available as Source data.
