Extended Data Fig. 1: Aggregation kinetics of αSyn variants at different pH and salt conditions with TEM images of the aggregates (if any) formed at the end point.
From: A short motif in the N-terminal region of α-synuclein is critical for both aggregation and function
a−h, ThT fluorescence assays at pH 4.5 or pH 7.5 of WT αSyn (a,b), ΔP1 (c,d), ΔP2 (e,f) and ΔΔ (g,h) at high (200 mM NaCl) or low (20 mM NaCl) ionic strength. Negative-stain TEM images of representative samples of the aggregates formed at the end point (100 h) are shown alongside each plot using the same color scheme. The fibril yield under each condition, determined by SDS−PAGE after centrifugation (Methods), is shown in Supplementary Table 1. Data for graphs are available as Source data.
