Extended Data Fig. 2: Establishment of Hsp27 KO HeLa cells and cellular expression levels of FUS, Hsp27 and αBc (control) by western blot.
From: Hsp27 chaperones FUS phase separation under the modulation of stress-induced phosphorylation
a, A fragment in Hsp27 exon 1 was deleted by using CRISPR/Cas9. We constructed two KO plasmids—sgHsp27-1 and sgHsp27-2. b, Western blot shows that plasmid sgHsp27-2 is efficient to suppress Hsp27 expression. sgNC is empty plasmid transfected in cells as control. c, A similar expression level of FUS in the Hsp27 KO and WT cells. d, Expression of endogenous (endo-Hsp27) and exogenous (myc-Hsp27) with/without stress (0.5 mM sodium arsenite). e, Expression levels of αBc with or without stress and Hsp27 overexpression. f, A similar expression level of FUS in cells with or without myc-Hsp27 overexpression.
