Extended Data Fig. 3: Hsp27 reverses the LLPS of FUS-LC, while exhibits no effect on FUS-RGG LLPS.
From: Hsp27 chaperones FUS phase separation under the modulation of stress-induced phosphorylation
a, Visualization of the reversion of Hsp27 to FUS-LC LLPS in test tubes and by DIC and fluorescence imaging. Scale bar: 10 μm. αBc is performed as a negative control. b, The LLPS of 150 μM FUS-LC was induced by decreasing the temperature to 4 °C, followed by the measurement of turbidities at OD600 nm. The molar ratios of Hsp27 are indicated. Error bars correspond to mean ± S.D. with n=3. c, αBc (negative control) exhibits no effect on FUS-LC LLPS. The test tubes contain 150 μM FUS-LC in the presence of 30 μM αBc at 25 °C and 4 °C, respectively. The DIC and fluorescence images show the liquid-like droplets formed by FUS-LC. Scale bar: 10 μm. d, Effect of Hsp27 on the LLPS of FUS-RGG. DIC images are shown with scale bars representing 10 μm. e, Turbidity measurement of 50 μM FUS-RGG in the presence of indicated ratios of Hsp27 at 4 °C. Error bars correspond to mean ± S.D., with n = 3. N. S. indicates not significant.
