Extended Data Fig. 8: Sedimentation velocity analysis of NαBc-Hsp27 and NHsp27-αBc, and the influence of Hsp27 ΔCTD-3D on FUS-LC LLPS.
From: Hsp27 chaperones FUS phase separation under the modulation of stress-induced phosphorylation
a, Sedimentation velocity analysis of NαBc-Hsp27 (20 μM) and NHsp27-αBc (17 μM). b, The influence of Hsp27 ΔCTD-3D on the phase transition of FUS-LC. The turbidity (OD600 nm) of 150 μM FUS-LC in the absence and presence of ΔCTD-3D at the indicated concentrations were monitored from 19 °C to 4 °C.
