Extended Data Fig. 1: Intracellular localization of endogenous Hsp27.
From: Hsp27 chaperones FUS phase separation under the modulation of stress-induced phosphorylation
a, Endogenous Hsp27 (endo-Hsp27, red) of HeLa cells localizes in the cytoplasm under normal condition. As treated with stress (0.5 mM sodium arsenite), endo-Hsp27 condenses in SGs. Cells were immunostained for DAPI (blue). Scale bar, 20 μm. b, HeLa cells expressing RFP-FUS were treated with stress (0.5 mM sodium arsenite). Co-localization of Hsp27 (purple) and overexpressed RFP FUS (red) in SGs are indicated with arrows. Cells were immunostained for G3BP1 (green) to mark SGs. Scale bar, 20 μm.
