Extended Data Fig. 5: Real-time monitoring of CHMP1B and IST1 membrane constriction and elongation.
From: Membrane constriction and thinning by sequential ESCRT-III polymerization
a-c, Still images representing deformation of two membrane tubes due to transverse flow of (a) buffer alone, (b) then 0.5 μM CHMP1B, (c) and a final addition of 0.5 μM IST1. Solid arrows in (a-c) highlight tubule locations. d-e, Contours of lower (d) and upper (e) membrane tubes extracted from panels (a-c) showing the extension of the tubes upon addition of CHMP1B and IST1.
