Extended Data Fig. 5: Fiber transfection fitting. | Nature Structural & Molecular Biology

Extended Data Fig. 5: Fiber transfection fitting.

From: Nucleation seed size determines amyloid clearance and establishes a barrier to prion appearance in yeast

Extended Data Fig. 5

Fiber transfection experiments from Fig. 1 were fit mathematically to determine the number of fibers required to induce a stable [PSI+] state. Shown are the observations (black) and fits for fibers of the prion domain of Sup35 assembled at 4 °C (Sc4) or at 37 °C (Sc37) in [pin-] (a, c) or [PIN+] (b, d) yeast strains to a model of one (red), two (green), or three (blue) fibers. Data shown are means and s.d. from at least two independent experiments performed with independent cultures.

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