Extended Data Fig. 2: Substrate polyubiquitination assays by APC/CCDH1, UBE2C, and UBE2S. | Nature Structural & Molecular Biology

Extended Data Fig. 2: Substrate polyubiquitination assays by APC/CCDH1, UBE2C, and UBE2S.

From: Ubiquitin chain-elongating enzyme UBE2S activates the RING E3 ligase APC/C for substrate priming

Extended Data Fig. 2

UBE2S extends Ub chains on substrates, CycBNTD* and Securin*, and increases the rate of substrate modification in a dose-dependent manner. Fluorescence scan of full SDS-PAGE gels used in (1b). * Represents contaminant present in substrate stock. Uncropped images are available as source data.

Source data

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