Extended Data Fig. 9: Phenotypes of S. aureus WT, ∆ltaA, and LtaA mutants.
From: Structure of a proton-dependent lipid transporter involved in lipoteichoic acids biosynthesis
a, Over-expression of LtaA mutants. S. aureus strain NCTC8325 growth on C+Y agar plates in the presence of 0.1 mM IPTG incubated at 37 °C and 5% CO2. b, Over-expression of LtaA mutants. S. aureus strain NCTC8325 growth on C+Y agar plates in the presence of 0.1 mM IPTG incubated at 37 °C under different pH conditions. LtaA WT represents ∆ltaA mutant complemented with wild type ltaA on pLOW vector; Ctrl vector indicates ∆ltaA mutant complemented with pLOW carrying a functionally unrelated gene as vehicle control; the other labels represent ∆ltaA mutant complemented with ltaA with corresponding point mutations. c, Transmission electron microscopy (TEM) images at low magnification showing the morphology of S. aureus NCTC8325 WT and ∆ltaA mutant.
