Extended Data Fig. 2: S. aureus LtaA crystallization.
From: Structure of a proton-dependent lipid transporter involved in lipoteichoic acids biosynthesis
a, SDS-PAGE of samples from different steps of a LtaA purification experiment. Purified protein after cleavage of the His10-tag was used for crystallization. b, Size exclusion chromatography profile of purified LtaA (Superdex 200 10/300 Increase). Gray arrow indicates column void. c, Representative X-ray diffraction images of a LtaA crystal before in situ annealing (left) and after in situ annealing (right). The difference in unit cell dimensions before and after in situ annealing demonstrate shrinking of the unit cell. d, Stereo view (wall-eyed) of the 2Fo-Fc electron density map of the 3.3Å structure of LtaA at 1.0σ level.
