Extended Data Fig. 4: Confirmation of TENT4-dependent regulation of RNA2.7 reporters.
From: Viral hijacking of the TENT4–ZCCHC14 complex protects viral RNAs via mixed tailing
a, Immunoprecipitation with anti-TENT4A was confirmed by western blotting in RNA2.7-transfected HEK293T cells. Normal mouse IgG (NMG) was used as a negative control. RNA2.7 transcript is shown with bar indicating the position of RT-qPCR amplicon. b-e, RNA levels of firefly reporters harboring b, subregions of RNA2.7 (n = 3 independent experiments), c, deletion constructs of fragment 1 (1–513) of RNA2.7 (n = 3 independent experiments), d, construct 1D (314-413), 1E (414–513) and stem-loop mutant 1E (n = 3 independent experiments) and e, construct SL2.7 (414–463) and controls (n = 3 independent experiments) in parental and TENT4 KO cells. mRNA levels of both renilla and control vector were used for normalization. Mean was calculated and Error bars represent SEM. *P < 0.05, **P < 0.01; two-sided t test. Uncropped blots for panel a and data for graphs in b-e are available as source data.
