Extended Data Fig. 4: Cryo-EM single-particle analysis of cGMP-unbound F403V V407A (VA) mutant TAX-4 reconstituted in lipid nanodiscs.
From: Mechanism of ligand activation of a eukaryotic cyclic nucleotide−gated channel
a, Gel filtration profile of cGMP-bound VA TAX-4 nanodisc sample. b, SDS-PAGE of cGMP-bound VA TAX-4 nanodisc sample used for cryo-EM. c, A representative motion-corrected micrograph. d, Fourier power spectrum of the micrograph shown in (c). e, Gallery of typical averages from 2D classification. f, Flow chart of cryo-EM image processing. g, Euler angle distribution of particles used in the final 3D reconstruction with C4 symmetry. h, Local resolution of the final density map reconstructed with C4 symmetry. i, Densities of the SF generated from the two half maps, contoured at 5σ and overlaid with the model. j, Gold-standard FSC curves of the final 3D reconstructions with C4 and C1 symmetry, respectively. k, FSC curves for cross-validation between maps and model. Black, model versus the summed map. Blue, model versus the half map that was used for model refinement (called ‘work’). Red, model versus another half map that was not used for model refinement (called ‘free’). Uncropped image for panel b is available as source data.
