Extended Data Fig. 3: Quality of cryo-EM data.
From: Structure of complete Pol II–DSIF–PAF–SPT6 transcription complex reveals RTF1 allosteric activation
a, Estimate of average resolution. The lines indicate the Fourier shell correlation (FSC) between the half maps of the reconstruction. FSC curves are shown for each deposited map. b, Angular distribution of particles from overall refinement (Map 1). Shading from blue to yellow indicates the number of particles at a given orientation. c, Complete EC* reconstructions as colored by local resolution as implemented in RELION 3.0. The overall refinement (Map 1) was subjected to local resolution calculations with an estimated B factor of −115 and −20. A B factor of −20 was applied to Map 2. Top and side views are shown.
