Extended Data Fig. 6: Native MS identifies a specific interaction between LmrP and PG phospholipid inside the binding pocket cavity.
From: An embedded lipid in the multidrug transporter LmrP suggests a mechanism for polyspecificity
Mass spectra recorded at increasing energies by modulating in-source trapping voltage values for a, LmrP and b, LmrP N116Y reconstituted in PE:PG nanodiscs (80:20). c, A plot of the relative fractional intensity of the single PG-bound LmrP peak versus the LmrP apo peak over increasing source voltage values. The individual mass spectra at each voltage value were transformed using the MaxEnt 1 deconvolution algorithm and the peak intensities of both bound and unbound species were extracted. The red arrow indicates the voltage value where no PG lipid is observed on the mutant anymore.
