Extended Data Fig. 6: Identification of inhibitory elements in 5’UTR that impair translation.
From: Decoding mRNA translatability and stability from the 5′ UTR
a, Flow cytometry scatterplots of HEK 293-Kb cells transfected with mRNA reporters enriched in the ribosome-free fractions (N1 – N8). b, HEK293-Kb cells were transfected with mRNA reporters or plasmids with sequence variants chosen from the ribosome-free fractions (N1 – N8), followed by RT-qPCR at indicated time points (n = 3 biological replicates; t test). Error bars indicate SEM. ** P < 0.01. c, Flow cytometry scatterplots of HEK 293-Kb cells transfected with mRNA reporters enriched in the ribosome-free fractions (N1 – N8) and stained with QUMA-1.
