Extended Data Fig. 7: RG4 in 5’UTR triggers mRNA decay in the P-body.
From: Decoding mRNA translatability and stability from the 5′ UTR
a, HEK293-Kb cells with specific decay factor knockdown were transfected with the N1 mRNA reporter, followed by RT-qPCR at indicated time points. (n = 3 biological replicates; t test). Error bars indicate SEM. * P < 0.05. b, Representative confocal images of HEK 293-Kb cells transfected with P1 or N1 mRNA reporters. The mRNA reporters were synthesized in the presence of Alexa Fluor-UTP and the transfected cells were co-stained with a DCP2 antibody. DNA was counter-stained with Hoechst 33342. Arrowheads indicate typical mRNA foci. Bar, 10 μm. Images are representative of at least 50 cells.
