Extended Data Fig. 1: Characterization of uORF reporters.
From: Decoding mRNA translatability and stability from the 5′ UTR
a, Basic design of the uORF reporter with the SIINFEKL sequence highlighted. 5 additional amino acids (LEQLE) are present, which permits processing of SIINFEKL from the same flanking amino acids regardless of the TIS sequence. b, Bar graphs show the 25D1 or GFP mean fluorescence of HEK293-Kb cells transfected with plasmids of uORF reporters. n = 2 biological replicates, Error bars indicate SEM. c, Representative flow cytometry scatterplots of HEK 293-Kb cells transfected with synthetic mRNA reporters with optimal or suboptimal AUG codons. d, A bar graph shows the ratio of 25D1/GFP in HEK 293-Kb cells transfected with synthetic mRNA or plasmid DNA. Error bars, mean ± s.e.m; n = 3 biological replicates.
