Extended Data Fig. 2: Establishing massively parallel uORF reporters.
From: Decoding mRNA translatability and stability from the 5′ UTR
a, Schematic of generating a library of RNA-based uORF reporters by PCR-amplification using primers composed of random 10-nt sequences upstream of the uORF. Pooled PCR products were utilized as templates for in vitro RNA synthesis followed by 5’ capping and 3’ polyadenylation. b, Comparison of sequence randomness for nucleotide oligos synthesized by different vendors. c, A histogram shows the distribution of read count on individual unique random sequences. d, A scatter plot shows the correlation of read counts between two biological replicates of original oligo sequences (Rho = 0.79, P < 2.2 × 10-16).
