Extended Data Fig. 1: Biophysics of the nanobody binding to RBD.
From: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2
(a) Raw sensorgrams for the H11 parent nanobody. (b) KD of the H11 parent nanobody. (c) H11-H4 binding to RBD-Fc, repeat of Fig. 2b. (d) H11-H4 binding to RBD-Fc. (e) Binding of analytes to ACE2-Fc immobilized on the chip. H11-D4 behaved identically to H11-H4 (Fig. 2c). (f) Binding of analytes to CR2022-Fc immobilized on the chip. H11-D4 behaved identically to H11-H4 (Fig. 2d).
