Extended Data Fig. 3: The addition of seed fibrils accelerates the aggregation of Aβ42.
From: Direct measurement of lipid membrane disruption connects kinetics and toxicity of Aβ42 aggregation
a, Monomeric Aβ42 was incubated at a concentration of 2 μM in the presence of 20 μM ThT at 37 °C under quiescent conditions in the presence of no (red), 0.5% (orange), 1% (light green), 2.5% (dark green), 10% (blue) and 20% (black) preformed fibrils. b, Global fits to the kinetic traces of the aggregation reaction. c, The half-times of the aggregation reaction with varying concentrations of seed fibrils. d, Drawing of the microscopic events of Aβ42 aggregation in the presence of the preformed fibrils that accelerate the aggregation. Addition of seed fibrils allows bypassing de novo formation of new aggregates by primary nucleation, favouring secondary nucleation.
