Extended Data Fig. 1: Preferred specimen orientation.
From: Structure and mechanism of B-family DNA polymerase ζ specialized for translesion DNA synthesis
a, Data collected at 0° stage angle resulted in disproportionally low number of classes for side-views of the ternary complex of Polζ depicted in the 2D class averages. This resulted in a ‘smeared 3D model’ as shown by the anisotropic 3DFSC plot. Scale bar = 123 Å. b, Data collected at a stage angle of 0° for the apo-state of Polζ also had preferred set of views as shown in the 2D class averages. The final construction was anisotropic as depicted by the directional FSC plot. Scale bar = 123 Å.
