Extended Data Fig. 3: Cryo-EM data collection and processing for Polζ apo state.
From: Structure and mechanism of B-family DNA polymerase ζ specialized for translesion DNA synthesis
a, Data were collected at a 40° tilt angle and processed in cryoSPARC to give a good distribution of particles (green) with different views depicted in the 2D class averages. The final 3D reconstruction displaying a FSC0.143 of 4.1 Å showed an isotropic map amenable for model building. Scale bar = 123 Å. b, Per- particle CTF refinement of the map improved the sphericity further as shown by the 3DFSC plot.
