Extended Data Fig. 1: Source of RBP ORFs, correlation between luciferase levels and RBP sizes, concordance between two luciferase systems, and correlation of reporter RNA and luciferase levels.
From: Large-scale tethered function assays identify factors that regulate mRNA stability and translation
a, Western dot blot analysis of transiently expressed MCP-V5-tagged RBP ORFs in HeLa cells using a V5 antibody. Blue circles denote negative controls (no plasmid), red circles denote positive controls (CNOT7-V5-MCP). The order of wells and fold changes over negative controls are listed in Extended Data Table 1. b, Distribution of known classical and nonclassical RNA-binding domains in RBPs represented in our library. c, Distribution of molecular categories for RNA-related functions of RBPs represented in our library. d, Scatter plot of RBP size and luciferase effect. R, Pearson correlation coefficient. e, Luciferase activities from two different reporter constructs. Bar graphs showing log2-fold changes of the activity of Renilla (top) or firefly (bottom) luciferase reporters in presence of the MCP-fusion ORFs over FLAG control. Each vertical line represents a tethered ORF. f, Scatter plot of luciferase activities from the two reporter constructs. Values are expressed as log2-fold changes of the mean luciferase activity in the presence of MCP-fusion ORFs over FLAG controls. R, Pearson correlation coefficient.
