Extended Data Fig. 5: Thermostability of the S protein constructs.
From: Controlling the SARS-CoV-2 spike glycoprotein conformation
a–c, SEC profile of the S-proteins. The dotted lines indicate the portion of the peak that was collected for further studies. The unmutated and u1S2q spikes were run on a Superose 6 Increase 10/300 column, and the rS2d spike was run on an analytical Superose 6 Increase 5/150 column. d–i, Unfolding profile curves obtained by intrinsic fluorescence measurements using Tycho NT. 6. d–f, show ratio between fluorescence at 350 nm and 330 nm. g-i, plot the first derivative of this ratio. j, Inflection temperatures for the S-proteins. Asterisk mark the inflection temperatures in (g).
