Extended Data Fig. 7: Binding of spike constructs to ACE-2 and RBD-binding antibody CR3022.

a, SPR sensorgrams of CR3022 antibody binding to unmutated (black line), u1S2q (blue line) or rS2d (red line) spike captured on a strepdavidin chip. b, SPR sensorgrams of ACE-2 (with C-terminal human Fc tag) to unmutated (black line), u1S2q (blue line) or rS2d (red line) spike captured on a strepdavidin chip. c, SPR sensorgrams of binding of unmutated (black line), u1S2q (blue line) or rS2d (red line) spike to ACE-2 (with C-terminal human Fc tag) captured on an anti-mouse Fc surface. The orange and magenta dotted lines are binding curves for rS2d following negative selection over a CR3022 or ACE-2 column, respectively. d, Representative NSEM micrographs of the flow-through after negative selection of the rS2d sample through an ACE-2 column (left) and CR3022 column (right). e, Top views and f, side views of 3D classes of particles from the dataset from the CR3022 column-eluted sample. C1 symmetry was used during classification. For comparison, images from cryo-EM maps low-pass filtered to 20 Å of the 1-RBD ‘up’ state (EMD-21457) and 3-RBD ‘down’ state (EMD-21452) are shown in figures g, top view and h, side view.