Extended Data Fig. 6: Motors adopt distinct conformations at the two cell poles in the same ∆cheY3 cell.

a, A tomographic section from one cell tip showed in panel b. Scale bar, 200 nm. (b) An overview of one intact ∆cheY3 cell. Scale bar, 1 µm. c, A tomographic section from another tip of the same cell in panel b. Scale bar, 200 nm. The motors at each cell tip were aligned separately, then focused refined to the C-ring. d-f, The motors from one cell tip have CCW conformation. g-t, The motors from another tip appear to adopt CW conformation. j-l, Averaged structure from motors located at one tip of five cells shows a better structure with CCW conformation. m-o, Averaged structure from motors located at another tip of five cells shows a better structure with CW conformation.