Extended Data Fig. 3: Representative HS AFM image sequences of the condensin holocomplex in the presence of ATP.
From: The condensin holocomplex cycles dynamically between open and collapsed states
a, b, Images were taking from movies acquired at 5 frames/s rate, from Supplementary Video 2 in a and Supplementary Video 3 in b (N = 6 molecules, 3 independent experiments, 2 independent protein purification, and 5,240 frames). Changes between O- and B-shapes were observed. O and B indicate O-shape and B-shape, respectively. Cartoons on the right of each image are for visual guidance. c, Number of distinguishable subunits in the globular domain (that is, excluding the hinge) (N = 5,240 frames from 6 movies). About 70% of the complexes featured 3 distinguishable subunits in the globular domain, and about 30% featured 4 subunits. The complex with 3 subunits likely is composed of a dimerized head domain and two heat-domains, while two head domains were resolved separately in the case of the complex with 4 subunits in the globular domain.
