Extended Data Fig. 5: Proteasomal degradation of GFP-tagged proteins by TRIM21 RING-nanobody fusion.
From: Target-induced clustering activates Trim-Away of pathogens and proteins
a-d, NIH3T3-Caveolin-1-GFP (a,b) and RPE-1-H2B-mEGFP-FKBP (c,d) cells were electroporated with mRNA encoding mCherry-T21R-vhhGFP4, incubated with either DMSO (control) or MG132 and imaged (a,c) and GFP fluorescence quantified (b,d) with the IncuCyte system. Time, hours (h) post-electroporation. Scale bars, 20 µm. Error bars show s.e.m. from n = 4 technical replicates. Source data for graphs are in Supplementary Data 1.
