Extended Data Fig. 3: Effects of WDR82 and ZC3H4 co-depletion on extragenic transcription in HeLa cells.
From: A first exon termination checkpoint preferentially suppresses extragenic transcription
a, The effects of WDR82, ZC3H4 or their combined depletion by siRNA transfection were measured on selected extragenic transcripts, as indicated. In co-depletion experiments, a double amount of siRNA was used, as indicated. The bar plots show the mean ± SD of n=4 biological replicates. The data were normalized on the housekeeping gene CDC25b. Light grey columns: 30pmol siRNA, dark grey columns, 60pmol siRNA. b, Depletion efficiency of WDR82 (left) and ZC3H4 mRNA (right) in individual and combined depletions. The bar plots show the mean ± SD of n=4 independent experiments.
