Extended Data Fig. 1: Activity analysis of purified LolCDE complex.
From: Structural basis for bacterial lipoprotein relocation by the transporter LolCDE
a, Purified LolCDE was assessed for ATPase activity in detergent or liposomes at different ATP concentrations. b, c, The relative ATPase activities of LolCDE were measured in the presence of AMP-PNP (b) or ADP (c). d, In vivo cellular viability of the LolCDE silencing strain (HD200313) was studied. The LolCDE gene in HD200313 is controlled under an araBAD promotor that can only be induced in the presence of arabinose (method). Insertion of a leaky LolCDE expressing plasmid was used to test mutations in the LolCDE gene. e, ATPase activity determination of LolCDE with insertion of tags. f, LolCDE in liposomes of E. coli polar lipids were treated with or without proteinase K to assess the reconstitution orientations by SDS-PAGE. Data in d, f are representative results from n = 3 independent experiments. Data in a-c, e represent mean ± s.d. (n = 3 independent experiments). Statistics for panels a-c, e are available as Source Data. Uncropped images for panel d, f are available as Source Data.
