Extended Data Fig. 2: Image-processing workflow for lipoprotein-bound LolCDE.
From: Structural basis for bacterial lipoprotein relocation by the transporter LolCDE
a, Cryo-EM microscope and selected two-dimensional class averages of cryo-EM particle images of lipoprotein-bound LolCDE. b, Scheme of three-dimensional classification, refinement of cryo-EM particle images, and the final 3D reconstitution of lipoprotein-bound LolCDE. c, Gold-standard FSC curves of the final cryo-EM maps of lipoprotein-bound LolCDE. d, The overall cryo-EM maps of lipoprotein-bound LolCDE are colored according to the local resolution. e, Cryo-EM maps with the atom model for individual transmembrane helices of LolC and LolE and the lipoprotein.
