Extended Data Fig. 3: Image-processing workflow for AMP-PNP-bound LolCDE in closed and open NBD conformation.
From: Structural basis for bacterial lipoprotein relocation by the transporter LolCDE
a, Typical cryo-EM microscope and selected two-dimensional class averages of cryo-EM particle images of AMP-PNP-bound LolCDE. b, Scheme of three-dimensional classification, refinement of cryo-EM particle images and final 3D reconstitution of AMP-PNP-bound LolCDE with closed LolD at 4.1Å and open LolD at 3.2Å. c, Gold-standard FSC curves of the final cryo-EM maps of AMP-PNP-bound LolCDE.
