Extended Data Fig. 2: CryoEM characterization of the B6-bound MERS-CoV S complex. | Nature Structural & Molecular Biology

Extended Data Fig. 2: CryoEM characterization of the B6-bound MERS-CoV S complex.

From: Structural basis for broad coronavirus neutralization

Extended Data Fig. 2

(a) Representative image of 1,146 cryoEM micrograph of the MERS-CoV S prefusion trimer bound to B6 Fab embedded in vitreous ice. Scale bar: 20 nm. (b) Selected reference-free 2D class averages. Scale bar: 20Å. (c) Fourier shell correlation curves for the reconstructions shown in panels (d) and (e). (d) Reconstruction obtained with all selected particles and applying C3 symmetry colored by local resolution. (e) Reconstruction obtained with a subset of particles obtained through focused classification to improve B6 resolvability colored by local resolution. (f) Zoomed-in view of the MERS-CoV stem bound to the B6 Fab. The position of MERS-CoV S residues 1226-1229 connecting the cryoEM model to the B6/MERS-CoV stem peptide crystal structure is indicated as dashed line.

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