Table 1 Cryo-EM data collection and refinement statistics
B6–MERS-CoV S (C3 map, post polishing) (EMD-23674, PDB 7M5E) | B6–MERS-CoV S (C1 map, before polishing) (EMD-23672) | |
|---|---|---|
Data collection and processing | ||
Magnification | 130,000 | 130,000 |
Voltage (kV) | 300 | 300 |
Electron exposure (e–/Å2) | 70 | 70 |
Defocus range (μm) | −0.5 to −3.0 | −0.5 to −3.0 |
Pixel size (Å) | 1.05 | 1.05 |
Symmetry imposed | C3 | C1 |
Initial particle images (no.) | 317,017 | 317,017 |
Final particle images (no.) | 144,792 | 32,687 |
Map resolution (Å) | 2.5 | 4.7 |
FSC threshold | 0.143 | 0.143 |
Refinement | ||
Model resolution (Å) | 2.7 | |
FSC threshold | 0.5 | |
Map sharpening B factor (Å2) | −67.7 | −153.2 |
Model composition | ||
Nonhydrogen atoms | 28,503 | |
Protein residues | 3,477 | |
Ligands | 135 | |
B factors (Å2) | ||
Protein | 11.61 | |
Ligand | 17.32 | |
R.m.s. deviations | ||
Bond lengths (Å) | 0.017 | |
Bond angles (°) | 1.358 | |
Validation | ||
MolProbity score | 0.86 | |
Clashscore | 1.33 | |
Poor rotamers (%) | 0.2 | |
Ramachandran plot | ||
Favored (%) | 98.09 | |
Allowed (%) | 99.91 | |
Disallowed (%) | 0.09 | |
