Extended Data Fig. 3: Electron density clearly shows covalently bound 3-keto-eicosanoyl-CoA.
From: The structural basis of fatty acid elongation by the ELOVL elongases
a-d, Electron density running along the catalytic tunnel. Final BUSTER 2mFo-DFc (blue mesh, contoured at 1σ) and omit mFo-DFc (green mesh, contoured at 2.5σ) electron density maps are overlaid on the final model. e, Comparison of a test refinement in which the imidazole groups of H150 and H181 were removed from the model (grey carbon protein atoms / palecyan ligand carbon atoms) and the final model (palecyan protein carbons / violet ligand carbon atoms). The BUSTER 2mFo-DFc (blue mesh, contoured at 1σ) and mFo-DFc (green mesh, contoured at 3σ) maps for the refined histidine-truncated model / unlinked acyl-CoA are shown. f, Comparison of various refined models (green carbons - protein only; palecyan - protein without H150/181 sidechain plus ligand; grey/violet - final model with covalently attached ligand).
