Extended Data Fig. 4: Sequence alignment and active site conservation of human ELOVL family members.
From: The structural basis of fatty acid elongation by the ELOVL elongases
a, Sequence alignment of human ELOVL1-7. The conserved histidine box (147HxxHH151) is highlighted by a blue box. Filled circles below alignment indicate residues with a proposed catalytic role (blue) and residues interacting with either the CoA (orange) or acyl (plum) portion of the substrate. Cysteines that form the disulfide bridge (C99-C231) between the TM2/3 and TM6/7 loops are indicated by stars. b, Conservation of active site tunnel. Molecular surface representation is coloured by amino acid conservation score calculated by CONSURF analysis60 of a diverse set of ELOVL1-7 family members. The various subregions of the tunnel are indicated (ADP / Pan from CoA and Acyl chain). Amino acid residues that form the binding tunnel are coloured according to region (pink, acyl; blue, catalytic site; orange CoA binding).
