Extended Data Table 1 Mutagenesis primer sequences
From: The structural basis of fatty acid elongation by the ELOVL elongases
Primer purpose | Primer sequence (5′ to 3′) | |
|---|---|---|
WT ELOVL7 | Forward | TTAAGAAGGAGATATACTATGGCCTTCAGTGATCTTAC |
Reverse | GATTGGAAGTAGAGGTTCTCTGCATTATCTTTGTTTTTGCAAG | |
H150A | Forward | TCATGTATTCGCTCATACCATCATGCCG |
Reverse | AGGAAAGTCACTTGGCTATTTTTC | |
H151A | Forward | TGTATTCCATGCTACCATCATGCC |
Reverse | TGAAGGAAAGTCACTTGG | |
H181A | Forward | TACAGCTGTAGCTGTAGTCATGTATTCC |
Reverse | TTTAGAAGGGCATGGAATG |
