Extended Data Fig. 6: Overview of cryo-EM data collected for mTRPV3 in cNW11 nanodiscs at 42 °C and 3D reconstruction workflow.
From: Structural mechanism of heat-induced opening of a temperature-sensitive TRP channel
Representative micrographs with example particles circled in yellow and reference-free 2D class averages in different orientations are shown. Three datasets were collected and joined after particle clean-up. All processing steps were done in Relion, except the 2D/3D particle clean-up that was done in cryoSPARC.
