Extended Data Fig. 3: The blocking and unloading model of loop extruder interactions does not produce all the features seen in the Hi-C map.

The parameter sweep was conducted for varying numbers of extruders and facilitated dissociation rates. The experimental data (for the strain with parS sites at both −94^o and −59^o) is shown on the top left of the figure. These contact maps were generated with the semi-analytical approach without making the shortest path approximation as described in Appendix 3 of Banigan et al, 2020³⁰ (also see Supplementary Notes 1-5). Notably, Lines 4 and 5 are missing in all of the plots with the blocking and unloading model - this is due to either traffic jams forming between extruders (for high numbers of extruders), or an insufficient loading rate (for low numbers of extruders) preventing the efficient formation of nested doublets and triplets.