Extended Data Fig. 9: FLCN-10 peptide inhibits LDHA activity and induces cell death in ccRCC cell lines.
From: The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect
a) Caki-1 cells treated for 2 h with FLCN-10 or FLCN-13 were blotted using anti-cleaved caspase-3 and anti-pTyr10-LDHA. b) LDHA activity in HEK293 cells following 2 h treatment with FLCN-10 peptide. c) d) Flow cytometric assessment of cell death in renal cell lines following 1 µM FLCN-10 for 1 or 2 h (Extended Data Fig. 9c) or 0.1 µM or 1 µM FLCN-10 treatment for 2 h (Extended Data Fig. 9d) as determined by Annexin V/Propidium iodide staining. Representative of three independent experiments. e) Immunoblot of whole cell lysates following overexpression of FLAG-tagged FLCN-WT, FLCN-M222A, or FLCN-F226A in normal HEK293 cells or the ccRCC cell lines 786-O, Caki-1 and Caki-2. f) HEK293 cells were treated with and without 1 µM Rhodamine B-labeled FLCN-10A. Scale bar = 20 µM. g) LDHA activity in Caki-1 cells following 2 h treatment with FLCN-10A peptide.
