Extended Data Fig. 10: Heterocyclic FLCN-10 peptide induces cell death in ccRCC cells.
From: The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect
a) Structural representation of head-to-tail cyclic-FLCN-10 peptide, created using MAESTRO (Schrodinger.com). b) Flow cytometric assessment of cell death in renal cell lines following 1 µM or 10 µM cyclic-FLCN-10 treatment as determined by propidium iodide staining. Representative of three independent experiments. c) Representative flow cytometry gating strategy. Cells were first stratified by size, followed by staining intensity with Annexin V-FITC and Propidium Iodide (Extended Data Fig. 9c, d). The PI intensity signal was converted into a histogram for presentation in Fig. 5a and Extended Data Fig. 10b.
