Extended Data Fig. 2: Inherent specificity of FLCN for binding to dimeric LDHA.
From: The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect
a) IP of endogenous FLCN from whole cell lysates of LDHA or LDHB knockout HAP1 cells. Immunoblotted with anti-FLCN antibody. b) Western blot of siRNA knockdown of FLCN in Fig. 1d. c) Tagged LDHA and LDHB subunits were consecutively immunoprecipitated and immunoblotted with anti-FLCN. d) Lysates from Extended Data Fig. 1c subjected to Native PAGE. Bar chart contains ratio of densitometry of largest LDHA oligomer:GAPDH (see Extended Data Fig. 1c; Unpaired Student’s t-test, n=3). Data shown as mean ± s.d. e) IP of FLCN from HEK293 cells subjected to Native PAGE. SE = short exposure of immunoblot, LE = long exposure of immunoblot.
